Composite

Part:BBa_I2048

Designed by: Bartholomew Canton   Group: iGEM06_MIT   (2007-06-08)


Positive and negative feedback regulated T7 RNAP expression system

This part is an intermediate version of a biological virtual machine. T7 RNAP expression is controlled by a T7 promoter, forming a positive feedback loop or autogene. LacI expression is controlled by the same promoter. The promoter is repressible by LacI leading to negative feedback. The combination of positive and negative feedback should lead to a moderate, stable level of T7 RNAP in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3872
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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